RNA polymerase II
The 28 review prompts from the notes on RNA polymerase II. Answer each in your head, then reveal it. Your grade decides when the card comes back.
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RNA polymerase II copies a DNA gene into blank, the molecule that ribosomes read.
What pays for each nucleotide that RNA polymerase II adds to the RNA?
In RNA polymerase II, the two largest subunits, blank, build the DNA cleft and the active site.
Which part of RNA polymerase II sits next to the RNA exit groove and binds the emerging transcript?
Complete RNA polymerase II has blank subunits: a ten-subunit core plus the Rpb4-Rpb7 pair.
Inside transcribing RNA polymerase II, the DNA-RNA hybrid is blank base pairs long.
Which state of RNA polymerase is an explicit target for new antibiotics, according to structures with streptolydigin?
Why does phage T7 RNA polymerase give a private expression channel in an E. coli cell?
In which translocation state can an NTP pair in the addition site of RNA polymerase II?
What does the trigger loop of RNA polymerase II do when a matching NTP pairs in the addition site?
Why does the trigger loop link NTP recognition to catalysis in RNA polymerase II?
Of the two Mg2+ ions in the active site of RNA polymerase II, which stays and which comes and goes each cycle?
Why must the trigger loop of RNA polymerase II open before pyrophosphate can leave?
In the Brownian ratchet model, RNA polymerase slides back and forth along the DNA by thermal motion, and blank catches it in the forward position.
How does RNA polymerase II remove a wrong base it has just added?
The factor blank rescues backtracked RNA polymerase II by helping it cut its RNA.
A skin cell and a nerve cell carry the same genome. What makes them differ, in terms of what RNA polymerase II copies?
One RNA polymerase II takes about blank to copy the human dystrophin gene once.
About how many RNA polymerase II molecules does one HeLa cell hold?
In a fast-growing yeast cell, blank of all RNA polymerase II transcription serves the genes for ribosomal proteins.
Where does the "train on a track" analogy for RNA polymerase II break down?
Over long human genes, RNA polymerase II copies about blank, about 63 nucleotides per second.
How did Singh and Padgett measure the speed of RNA polymerase II over long human genes?
About what transcription error rate per base was measured across all mRNA of the worm Caenorhabditis elegans?
How did Gout and colleagues tell real transcription errors apart from errors made while copying and sequencing the RNA?
According to Thomas and colleagues, what delay, more than the slow addition of the wrong base itself, lets RNA polymerase II tell right from wrong?
How did Wang and colleagues show that RNA polymerase II rejects 2′-deoxy NTPs mainly at the chemical step, not at binding?
Which model of RNA polymerase movement did optical-trap force-velocity data favour over a power stroke tied to pyrophosphate release?
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